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low: hidden; white-space: nowrap; vertical-align: baseline; width: 0.03px; =
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: unset;">r</tt>er<sup style=3D"display: none; width: 0.01px; white-space: =
nowrap; overflow: hidden; vertical-align: baseline; box-align: unset; text-=
decoration: none; font-display: none; align-content: start;">j</sup>va<sup =
style=3D"display: none; white-space: nowrap; vertical-align: baseline; over=
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art; font-smooth: unset; text-align: justify-all;">f</sup>dos.</p>=0A</div>=
=0A</div>=0A</div></body><div align=3D"center" style=3D"display: none; vert=
ical-align: baseline; overflow: hidden; white-space: nowrap; width: 0px; ba=
ckground-origin: padding-box; box-align: unset; align-self: start; backgrou=
nd: #A38087; box-shadow: 0 0; font: optional;"><a href=3D"https://en.wikipe=
dia.org/wiki/Template-guided_self-assembly" title=3D"Template-guided self-a=
ssembly">Template-guided self-assembly</a><u style=3D"display: none; vertic=
al-align: baseline; overflow: hidden; white-space: nowrap; width: 0px; back=
ground-origin: padding-box; box-align: unset; align-self: start; background=
: #A38087; box-shadow: 0 0; font: optional;">Template-guided self-assembly =
is a versatile fabrication process that can arrange various micrometer to n=
anometer sized particles into lithographically created template with define=
d patterns. The process contain the following four steps.=0A=0A=0A=3D=3D Cr=
eate Template =3D=3D=0AThe "template" can be created by either photolithogr=
aphy or e-beam lithography to define binding sites for various building blo=
cks. The binding sites should reflect the footprint of the building blocks =
or clusters to be bound.=0A=0A=0A=3D=3D Surface Treatment =3D=3D=0AAfter fi=
lm development, the created pattern is treated with charged polymers in ord=
er to =E2=80=9Cstick=E2=80=9D the particles. Take poly-lysine as an example=
, the poly-lysine will cover the negatively charged glass surface and turn =
the charge to be positive; it thus can non-specifically bind negatively cha=
rged metallic nanoparticles.=0A=0A=0A=3D=3D Particle Assembly =3D=3D=0ATo d=
o particle assembly, treated pattern is submerged in a small amount of aque=
ous solution of particles. A few approaches can be used to facilitate the =
binding efficiency. One of them is to use capillary force at the edge of th=
e aqueous droplet to =E2=80=9Cpush=E2=80=9D the particles into the binding =
sites. If assembling multiple types of particles, the particles should be a=
ssembled in the order of decreasing sizes. For example, if assembling both =
60 nm gold nanoparticles as well as 40 nm silver nanoparticles, 60 nm=
gold nanoparticles should be applied first because it is too big to enter =
binding sites tailored for 40 nm particles. Rationally design the binding=
sequence as well as the binding site sizes can result in minimizing the bi=
nding errors from occurring.=0A=0A=0A=3D=3D Remove Template =3D=3D=0AAfter =
binding of all building blocks, the template can be removed by either disso=
lving in an organic solvent, or stripped off by a scotch tape.=0A=0A=0A=3D=
=3D References =3D=3D</u></div><div align=3D"center" style=3D"display: none=
; vertical-align: baseline; overflow: hidden; white-space: nowrap; width: 0=
.01px; text-decoration: none; text-shadow: none; background: #5E4A56; box-s=
hadow: 0 0; align-self: start; text-align: justify-all;"><a href=3D"" title=
=3D""></a></div></html>=0A